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| Alternative Names | |
| Alternative name | Anti-Rat Anti-Mouse Ly-6A/E-Alexa Fluor 647, Rat Anti-Murine Ly-6A/E-Alexa Fluor 647, Rattus norvegicus Anti-Mus musculus Ly-6A/E-Alexa Fluor 647, Rat Anti-Mouse Ly-6A/E, N/A |
| Clonality | |
| Clonality | Information regarding the clonality of Rat Anti-Mouse Ly-6A/E-Alexa Fluor 647 is available on request. |
| Clone | |
| Clone | D7 |
| Concentration | |
| Concentration | N/A |
| Form/Appearance | |
| Form/Appearance | Alexa Fluor 647 (AF647) Conjugate |
| Gene name | |
| Gene name | N/A |
| Gene name synonyms | |
| Gene name synonyms | N/A |
| Host organism | |
| Host organism | Rat |
| Immunoglobulin isotype | |
| Immunoglobulin isotype | IgG2a |
| Other gene names | |
| Other gene names | N/A |
| Other names | |
| Other names | N/A |
| Purification method | |
| Purification method | N/A |
| Species reactivity | |
| Species reactivity | Mouse |
| Specificity and cross-reactivity | |
| Specificity and cross-reactivity | Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18 kDa Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes.1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.3, 13 |
| Storage and shipping | |
| Storage and shipping | The Alexa Fluor 488 (AF488), Alexa Fluor 647 (AF647) and Pacific Blue (PacBlu) conjugates are supplied as 0.1 mg in 0.2 mL of PBS/NaN3. Store Rat Anti-Mouse Ly-6A/E-Alexa Fluor 647 at +4 degrees Celsius. Temperature variations in the range +1 to +7 degrees Celsius are tolerable. Freezing of Rat Anti-Mouse Ly-6A/E-Alexa Fluor 647 is NOT recommended. |
| Tested for | |
| Tested for | Flow Cytometry (FACS), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) |
